Organization of the mitotic chromosome.

نویسندگان

  • Natalia Naumova
  • Maxim Imakaev
  • Geoffrey Fudenberg
  • Ye Zhan
  • Bryan R Lajoie
  • Leonid A Mirny
  • Job Dekker
چکیده

Mitotic chromosomes are among the most recognizable structures in the cell, yet for over a century their internal organization remains largely unsolved. We applied chromosome conformation capture methods, 5C and Hi-C, across the cell cycle and revealed two distinct three-dimensional folding states of the human genome. We show that the highly compartmentalized and cell type-specific organization described previously for nonsynchronous cells is restricted to interphase. In metaphase, we identified a homogenous folding state that is locus-independent, common to all chromosomes, and consistent among cell types, suggesting a general principle of metaphase chromosome organization. Using polymer simulations, we found that metaphase Hi-C data are inconsistent with classic hierarchical models and are instead best described by a linearly organized longitudinally compressed array of consecutive chromatin loops.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

CHROMOSOME COUNTS OF SOME ANGIOSPERM SPECIES FROM IRAN (III)

Original meiotic or mitotic chromosome counts are presented for 17 species in 12 families of angiosperms from Iran. Chromosome counts for Amberboa nana, n=16 (Asteraceae) and Camelina rumelica subsp. transcapica, n=13 (Brassicaceae) are repoted for the first time. Chromosome numbers for six species including: Rhagadiolus stellatus (n=5), Campanula raponcoloides (n=34), Campanula trachelium (n=1...

متن کامل

Karyotypic Study and Chromosome Evolution in Some Iranian Local Onion Populations

Abstract A karyotypic study was performed on 12 Iranian local onion (Allium cepa L.) populations. A number of mitotic cells at metaphase stage for each population were prepared. Chromosomes of suitable mitotic cells were counted and various parameters, including long arm (L), short arm (S), total length of chromosome (TL), relative length of chromosome (RL), arm ratio (AR), r-value, total chro...

متن کامل

Initiation of Ageing Process by Meiotic and Mitotic Recombination within the Ribosomal DNA Genes in Saccharomyces cerevisiae

In the budding yeast of Saccharomyces cerevisiae the tandem repeated of rDNA genes are located onchromosome XII, which is in the nucleolus. There are different types of proteins in the nucleoluskeleton,silencing proteins have got important role in nucleolus.It is shown that meiotic recombination between nonsister chromatids in the rDNA genes are stronglysuppressed, and s...

متن کامل

New chromosome counts in some plant species from east and south-east Iran

In this study, we investigated the chromosome numbers of some endemic species distributed in East and South-East Iran. The mitotic chromosome counts were presented for the first time for six species in three angiosperm families including: Linaria iranica (2n=12), Nanorrhinum campyloceras (2n=18), Chaenorhinum grossecostatum (2n=24) (Plantaginaceae), Gaillonia bruguieri (Rubiaceae) (2n=22), Nepe...

متن کامل

Allelopathy and Anti-mitotic Effects of Cuscuta campestris and Cuscuta monogyna Extracts on Plant Cell Division

Some natural compounds of plants including phenols and alkaloids induce mitosis depressive that are blocked cell division. Field dodder (Cuscuta campestris Yunck.) and eastern dodder (Cuscuta monogyna Vahl.) are the most widespread obligatory parasitic species. In the present study, end of summer 2015, field dodder and eastern dodder are collected from Alhagi maurorum Medik. host and Vine (Viti...

متن کامل

Induction of premature chromosome condensation (PCC) by calyculin A for biodosimetry

Background: Premature chromosome condensation (PCC) is a method for interphase chromosome analysis in biodosimetry. This study was performed to verify the usefulness of PCC induced by calyculin A in human peripheral blood lymphocytes (pbl) for biological dosimetry and possible construction of dose-response curve. Materials and Methods: Peripheral blood was obtained from a healthy donor and expo...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Science

دوره 342 6161  شماره 

صفحات  -

تاریخ انتشار 2013